Figure 4.

(a) Basal insulin secretion at 2.8 mM glucose (n = 5–6); (b) Stimulated insulin secretion at 16.7 mM glucose (n = 5–6); (c) Fold stimulation of insulin secretion calculated as the ratio between stimulated and basal secretion (n = 5–6); (d) Chronic insulin secretion to the culture media corresponding to the last 72 h in culture (n = 5–6); (e) Intracellular insulin content of islet microtissues (n = 5–6); (f) Intracellular ATP content of islet microtissues (n = 5–6). Outliers were detected with ROUT’s test (Q = 5%). Statistical analysis was performed with one-way ANOVA for each transduction method separately. Dunnett’s multiple comparisons tests against the untransduced control of each condition are shown: *** p < 0.001, ** p < 0.01, * p < 0.5.