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Representative overlay MRM ion-chromatograms of isobaric eicosanoids that share some fragments, as well as retention times, were distinguished via specific MRMs: (a–c) blank charcoal-stripped surrogate matrix of selected analytes, (d–f) spiked in charcoal-stripped surrogate matrix of selected analytes (0.5 g/mL), and (g–i) healthy colon sample of selected analytes.
