Figure 3.

Importance of heparan sulfate on infection and transduction. (A) Infection of heparin pretreated HAdVs on A549 cells. The number of infected cells were quantified by immunofluorescence and shown as % infection of untreated HAdVs. (B) Transduction of HAdV-F41 GFP vector on CHO cells expressing or lacking heparan sulfate (HS) and (C) transduction of heparin pretreated HAdV-F41 GFP vector on CHO-K1 cells. The parental cell line CHO-K1 expresses glycosaminoglycans and CHO-ΔHS specifically lacks heparan sulfate but expresses other glycosaminoglycans. The number of infected/transduced cells were quantified by immunofluorescence and shown as % infection of untreated A549 cells. All experiments were performed three times with duplicate samples in each experiment. Error bars represent mean ± SEM. *** P < 0.001 versus control.