Figure 7.

Effect of GAGs on fiber knob binding to and infection of HuTu80 cells: Binding of (A) heparin or (B) chondroitin sulfate mix or (C) hyaluronic acid pretreated HAdVs on HuTu80 cells. Binding was determined by geometric mean of fluorescence on a FACSLSRII flow cytometer and shown as % binding of untreated fiber knobs. Infection of (D) heparin or (E) chondroitin sulfate mix or (F) hyaluronic acid pretreated virions to HuTu80 cells. The number of infected cells were quantified by immunofluorescence and shown as % infection of untreated HAdVs. All experiments were performed three times with duplicate samples in each experiment.