Table 2.
Primer sequences for quantitative real-time PCR
| Gene name1 | Primers sequence (5′–3′) | GenBank accession number | |
|---|---|---|---|
| GPX1 | Forward | AAGTGCCAGGTGAACGGGAAGG | NM_001277853.2 |
| Reverse | AGGGCTGTAGCGGCGGAAAG | ||
| SOD1 | Forward | GGTGCTCACTTTAATCCTG | NM_205064.1 |
| Reverse | CTACTTCTGCCACTCCTCC | ||
| HMOX1 | Forward | CTCAAGGGCATTCATTCG | NM_205344.1 |
| Reverse | ACCCTGTCTATGCTCCTGTT | ||
| NRF2 | Forward | ATCACCTCTTCTGCACCGAA | NM_205117.1 |
| Reverse | GCTTTCTCCCGCTCTTTCTG | ||
| ZO-1 | Forward | CCAAAGACAGCAGGAGGAGA | XM_015278981.1 |
| Reverse | TGGCTAGTTTCTCTCGTGCA | ||
| OCLN | Forward | TCATCCTGCTCTGCCTCATCT | NM_205128.1 |
| Reverse | CATCCGCCACGTTCTTCAC | ||
| CLDN1 | Forward | GAGGATGACCAGGTCAAGAAG | NM_001318434.1 |
| Reverse | TGCCCAGCCAATGAAGAG | ||
| MUC2 | Forward | CATTCAACGAGGAGAGCTGC | NM_001318434.1 |
| Reverse | TTCCTTGCAGCAGGAACAAC | ||
| AvBD1 | Forward | GAGTGGCTTCTGTGCATTTCTG | NM_204993.1 |
| Reverse | TTGAGCATTTCCCACTGATGAG | ||
| MYD88 | Forward | GAAGCAGCGTTTGGGAGTG | NM_001030962.4 |
| Reverse | AGCATTACCAGGGCTGAGTT | ||
| TLR4 | Forward | AGTCTGAAATTGCTGAGCTCAAAT | NM_001030693.1 |
| Reverse | GCGACGTTAAGCCATGGAAG | ||
| β-Actin | Forward | GAGAAATTGTGCGTGACATCA | NM_205518 |
| Reverse | CCTGAACCTCTCATTGCCA |
1GPX1, glutathione peroxidase 1; SOD1, superoxidate dismutase 1; HMOX1, hemoxygenase 1; NRF2, nuclear factor erthyroid 2-related factor 2; ZO-1 zona occludin 1; OCLN, occludin; MUC2, mucin 2; AvBD1, avian beta defensin 1; MYD88, myeloid differentiation factor 88; TLR4 toll-like receptor 4.