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. 2021 Feb 13;10(2):286. doi: 10.3390/antiox10020286

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of gallic acid (a) and rutin (b) on gene expressions of mitochondrial functions with SIRT1 siRNA transfection in C2C12 myotubes. C2C12 myotubes were transfected with SIRT1 siRNA by using Lipofectamine^® RNAiMAX reagent. After transfection, cells were treated with 25 μM of gallic acid (3 and 12 h) or rutin (1 and 9 h) at 37 °C in a humidified 5% CO₂ incubator. The reported values are the means ± SD (n = 3). Mean values with different letters were significantly different (p < 0.05).