Figure 4.

miR-146b-5p inhibitor relieves the constraint effects on cell proliferation, migration and invasion, as well as reduces the effect on apoptosis in SW1990 and AsPC-1 cells caused by SNHG7 silencing. SW1990 and AsPC-1 cells were transfected with sh-control, sh-SNHG7, sh-SNHG7 + inhibitor-control or sh-SNHG7 + miR-146b-5p inhibitor. Expression of miR-146b-5p was measured in (A) SW1990 and (B) AsPC-1 cells by reverse transcription-quantitative PCR. Cell viability was assessed in (C) SW1990 and (D) AsPC-1 cells via MTT assay. Apoptotic rate was evaluated in (E) SW1990 and (F) AsPC-1 cells via flow cytometry. (G) Migratory and (H) invasive abilities were detected via Transwell assay. ^*P<0.05 vs. the respective normal groups. SNHG7, small nucleolar RNA host gene 7; miR, microRNA; sh, short hairpin RNA; OD, optical density.