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. 2021 Feb 24;21(4):398. doi: 10.3892/etm.2021.9829

Figure 5.

Figure 5

Robo1 negatively interacts with miR-146b-5p. (A) Complementary binding sites between miR-146b-5p and Robo1 3'UTR, and the MUT sequences of Robo1 3'UTR. Luciferase activity of Robo1 3'UTR WT or Robo1 3'UTR MUT reporter in (B) SW1990 and (C) AsPC-1 cells transfected with miR-146b-5p mimics or miR-control was evaluated via dual-luciferase reporter assay. (D) mRNA and (E) protein expression levels of Robo1 were examined in SW1990 and AsPC-1 cells transfected with vector and Robo1. (F) mRNA and protein expression levels of Robo1 in (G) SW1990 and (H) AsPC-1 cells transfected with miR-control, miR-146b-5p, inhibitor-control or miR-146b-5p inhibitor were detected by reverse transcription-quantitative PCR and western blotting, respectively. (I) Correlation between Robo1 and miR-146b-5p was assessed with a Pearson test. *P<0.05 vs. their respective normal groups. 3'UTR, 3'untranslated region; MUT, mutant; WT, wild-type; miR, microRNA; Robo1, roundabout homolog 1; SNHG7, small nucleolar RNA host gene 7.