Figure 7.

SNHG7 silencing downregulates Robo1 expression by targeting miR-146b-5p. (A) Correlation between Robo1 and SNHG7 was determined by Pearson test. SW1990 and AsPC-1 cells were transfected with sh-control, sh-SNHG7, sh-SNHG7 + inhibitor or sh-SNHG7 + miR-146b-5p inhibitor. (B) mRNA expression of Robo1 was measured by reverse transcription-quantitative PCR. Protein expression of Robo1 was detected in (C) SW1990 and (D) AsPC-1 cells via western blotting. ^*P<0.05 vs. the respective normal groups. miR, microRNA; Robo1, roundabout homolog 1; SNHG7, small nucleolar RNA host gene 7; sh, short hairpin RNA.