Table 1.
Sense and antisense primer sequences used for real-time quantitative PCR.
| Gene | 5′ Primer (5′-3′)—Sense | 3′ Primer (5′-3′)—Antisense |
|---|---|---|
| 36B4 | TAAAGACTGGAGACAAGGTG | GTGTACTCAGTCTCCACAGA |
| 18S | GAGAGGGAGCCTGAGAAAC | GGCCTGCTTTGAACACTC |
| Lipe | GGGAGGGCCTCAGCGTTCTCACA | ATAGCACGGAGCTGGGTGAGGG |
| Fabp4 | AAGGTGAAGAGCATCATAACCCT | TCACGCCTTTCATAACACATTCC |
| Slc2a4 | CATTCCCTGGTTCATTGTGG | GAAGACGTAAGGACCCATAGC |
| AdipoQ | GCAGAGATGGCACTCCTGGA | CCCTTCAGCTCCTGTCATTCC |
| Cebpa | CGCAAGAGCCGAGATAAAGC | CAGTTCACGGCTCAGCTGTTC |
| Pnpla2 | GGTCCTCTGCATCCCTCCTT | CTGTCCTGAGGGAGATGTC |
| Lpl | GGCCAGATTCATCAACTGGAT | GCTCCAAGGCTGTACCCTAAG |
| Fasn | AGAGGCTTGTGCTGACTTCC | GTGGCTTCGGCGATGAGAG |
| Acaca | GAGAGGGGTCAAGTCCTTCC | ACATCCACTTCCACACACGA |
| Dgat1 | AGAGGTGTTGGGAGGATCTG | GTCTGAGTGGGTGGCAGGT |
| Lpin1 | TGATGTGGTGTTCAGTGTCACT | TCGTTGACCCAGTGCAGGTA |
| Nrf1 | CGCAGCACCTTTGGAGAA | CCCGACCTGTGGAATACTTG |
| Pparg2 | GCATCAGGCTTCCACTATGGA | AAGGCACTTCTGAAACCGACA |
| Cebpb | GCAAGAGCCGCGACAAG | GGCTCGGGCAGCTGCTT |
| Ppargc1a | ATCTACTGCCTGGGGACCTT | ATGTGTCGCCTTCTTGCTCT |
| Tfam | GGAATGTGGAGCGTGCTAAAA | TGCTGGAAAAACACTTCGGAATA |
| Ucp1 | CACCTTCCCGCTGGACACT | CCCTAGGACACCTTTATACCTAATGG |
| Prdm16 | CCACCAGCGAGGACTTCAC | GGAGGACTCTCGTAGCTCGAA |
| Cidea | ATCACAACTGGCCTGGTTACG | TACTACCCGGTGTCCATTTCT |
| Cited1 | CGCTTCGTCCGTACCTCAGCT | CAGCTGGGCCTGTTGGTCTC |
| Tmem26 | GAAACCAGTATTGCAGCACCC | CCAGACCGGTTCACATACCA |
| Tbx1 | CGAATGTTCCCCACGTTCCA | GTGTACTCGGCCAGGTGTAG |
| Fgf21 | CGTCTGCCTCAGAAGGACTC | TCTACCATGCTCAGGGGGTC |
| Dio2 | AATTATGCCTCGGAGAAGACCG | GGCAGTTGCCTAGTGAAAGGT |
| Pparalfa | TCGGACTCGGTCTTCTTGAT | TCTTCCCAAAGCTCCTTCAA |
| Bmp7 | CCTGTCCATCTTAGGGTTGC | GCCTTGTAGGGGTAGGAGAAG |
| Srebf1 | CACTCCCTCTGATGCTACGG | CTTGTTTGCGATGTCTCCAG |
| Cpt1 | TGTCCAAGTATCTGGCAGTCG | CATAGCCGTCATCAGCAACC |
36B4: Acidic ribosomal phosphoprotein P0; 18S: 18 S ribosomal RNA; Lipe (Hsl): hormone sensitive type; Fabp4: Fatty Acid Binding Protein 4; Slc2a4 (Glut-4): glucose transporter 4; AdipoQ: adiponectin; Cebpa (C/EBP-α): CCAAT/enhancer-binding protein alpha; Pnpal2 (Atgl): adipocyte triacylglycerol lipase; Lpl: Lipoprotein lipase; Fasn: fatty acid sinthase; Acaca (Acc1): Acetyl-CoA carboxylase; Dgat1: Diacylglycerol acyltransferase 1; Lpin1: lipin 1; Nrf1: Nuclear Respiratory Factor 1; Pparg2 (Ppar-γ2): Peroxisome proliferator-activated receptor gamma; Cebpb (C/EBP-β): CCAAT/enhancer-binding protein beta; Ppargc1a (Pgc1-α): Peroxisome Proliferator-Activated Receptor Gamma Coativator 1-alpha; Tfam: Transcription Factor A, Mitochondrial; Ucp1: Uncoupler protein 1; Prdm16: PR-domain containing 16; Cidea: Cell Death Inducing DFFA Like Effector A; Cited1: Cbp/P300 Interacting Transactivator With Glu/Asp Rich Carboxy-Terminal Domain 1; Tmem26: Transmembrane Protein 26; Tbx1: T-Box Transcription Factor 1; Fgf21: Fibroblast Growth Factor 21; Dio2: Iodothyronine Deiodinase 2; Pparalfa: Peroxisome Proliferator Activated Receptor Alpha; Bmp7: Bone Morphogenetic Protein 7; Srebf1: Sterol Regulatory Element Binding Transcription Factor 1; Cpt1: carnitine palmitoyltransferase 1.