Figure 1.

(A) Multiplex analysis of two ligands (thrombin, ATP) by 6-carboxyfluorescein (FAM)-functionalized thrombin aptamer and carboxy-X-rhodamine (ROX)-functionalized ATP aptamer using graphene oxide as an active support. The fluorescence of the respective fluorophores switches on after treatment with the ligands due to the desorption of the respective aptamer-ligand complexes from the graphene oxide. (B) Time-dependent fluorescent changes in the solution: Panel I—in the absence of ligands, Panel II—in the presence of thrombin, Panel III—in the presence of ATP, IV—in the presence of ATP and thrombin. a corresponds to the fluorescence of ROX and b of FAM. Reproduced with permission from ref. [90]. Copyright 2012 American Chemical Society. Arrows: the arrows correspond to typical excitation and emission arrows.