Table 1.
Secondary structure content derived from HEWL samples by the deconvolution of attenuated total reflectance—Fourier transform infrared spectroscopy (ATR-FTIR) spectra in the Amide I region and literature data derived from X-ray diffraction [11].
HEWL Sample | β-Sheet | Aggregation β-Sheet | α-Helix | Random Coil + Turn | Error |
---|---|---|---|---|---|
native, H2O | 10.3 | 8.6 | 41.7 | 39.4 | 1 |
0 h, 90% ethanol | 10.5 | 29.7 | 21.8 | 38 | 0.8 |
24 h, 90% ethanol | 10.1 | 34.7 | 22.8 | 32.4 | 0.6 |
96 h, 90% ethanol | 10.2 | 35.1 | 21.8 | 32.9 | 1.1 |
60 days, 90% ethanol | 10.3 | 38.2 | 19.6 | 31.9 | 0.8 |
X-ray diffraction | 18.7 | / | 42.6 | 38.7 | / |