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. 2021 Feb 22;21(4):383. doi: 10.3892/etm.2021.9814

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Copyright: © Min et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Live, dead and merged images of stem cell spheroids. (A) Live, dead and merged images of stem cell spheroids on day 1. Scale bar, 100 µm. (B) Live, dead and merged images of stem cell spheroids on day 3. Scale bar, 100 µm. (C) Live, dead and merged images of stem cell spheroids on day 5. Scale bar, 100 µm. (D) Live, dead and merged images of stem cell spheroids on day 7. Scale bar, 100 µm. (E) Cellular viability was assessed using a Cell Counting Kit-8 assay on days 1, 3, 5 and 7. ^*P<0.05 vs. IGF-2 at 0 ng/ml on day 5; ^**P<0.05 vs. IGF-2 at 0 ng/ml on day 7. IGF-2, insulin-like growth factor 2.