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. 2021 Feb 13;26(4):995. doi: 10.3390/molecules26040995

Figure 9.

Figure 9

P01F08 induces Bcl-2 dependent apoptosis. Jurkat cells overexpressing Bcl-2 and corresponding vector control cells were treated with 2.5 µM staurosporine (STS), 50 µM Etoposide, and 10 µM P01F08 for 24 h. (A) Apoptosis-related DNA degradation was detected via flowcytometric measurement of propidium iodide stained hypodiploid nuclei. Mean and SD of three independent experiments performed in triplicates are depicted. (B) Representative immunoblot of three independent experiments of cleavage of the caspase-3 substrate poly(ADP-ribose) polymerase 1 (PARP1; full-length 116 kDa, cleaved form 85 kDa) as an indicator for apoptotic cell death. anti-Tubulin (α-Tub) served as a loading control.