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. 2021 Feb 14;22(4):1895. doi: 10.3390/ijms22041895

Figure 3.

Figure 3

LTAP regulates skin moisturizing and aging factors through yes-associated protein (YAP). (A) HaCaT cells, NHEKs, and HDFs were exposed to LTAP for 1, 3, or 5 min, and further incubated for 24 h. Cells were harvested and the levels of phosphorylated YAP (pYAP), and YAP were evaluated by Western blotting. β-actin was used as a loading control. (B) HaCaT cells were treated with either 5 or 10 μM verteporin (VP), and further incubated for 24 h. The levels of pYAP and YAP were evaluated by Western blotting. Numerical values on the blots represent DU. The control was normalized to 1 DU. All data represent three independent experiments. (C) The expression of YAP, TGM, FLG, IVL and HAS-1, 2 in HaCaT cells was analyzed by qRT-PCR. (D) HDFs were treated with either 5 or 10 μM VP, and further incubated for 24 h. Cells were harvested for mRNA isolation, and expression of YAP, TGF-β, VEGF, COL1A1 and COL3A1 was determined by qRT-PCR. Data represent the mean ± SD. ** p < 0.01, *** p < 0.001. All data represent three independent experiments.