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. 2021 Feb 14;22(4):1895. doi: 10.3390/ijms22041895

Figure 4.

Figure 4

Skin barrier and moisturizing factors are mediated by YAP. (A,B) HaCaT cells were transfected with a plasmid overexpressing either green fluorescent protein (GFP; a control) or YAP, and incubated for 24 h. Cells were harvested and the expression of pYAP, YAP, FLG, and IVL was evaluated by Western blotting and the expression levels of YAP, FLG, and HAS-1, 2 were determined by qRT-PCR. For Western blotting, β-actin was used as a loading control. (C,D) HaCaT cells were transfected with control small interfering RNA (siRNA) or YAP siRNA, and further incubated for 24 h. The expression of pYAP, YAP, FLG, and IVL was determined at the protein level, and the expression of YAP, FLG and HAS-1, 2 was analyzed at the mRNA level. Numerical values on the blots represent DU. The control was normalized to 1 DU. Data are the means ± SD and represent three independent experiments. ** p < 0.01, *** p < 0.001.