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. 2021 Feb 14;22(4):1895. doi: 10.3390/ijms22041895

Figure 7.

Figure 7

The YAP inhibitor VP decreases melanogenesis-related gene expression. (A) B16F10 cells and NHEMs were exposed to LTAP for 1, 3, or 5 min, and incubated further for 24 h. Cells were harvested and the levels of pYAP and YAP were evaluated by Western blotting. β-actin was used as a loading control. (B,C) B16F10 cells were treated with VP (5 or 10 μM), and further incubated for 24 h. Cells were harvested and the expression of pYAP, YAP, MC1R, and MITF was evaluated by Western blotting. Expression levels of MC1R, MITF, protein melan-A (MART-1), SRY (sex determining region Y)-box 10 (SOX10), and paired box gene 3 (PAX3) were measured by qRT-PCR. Data represent the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Numerical values on the blots represent DU. The control was normalized to 1 DU. All data represent three independent experiments.