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. 2021 Feb 14;22(4):1895. doi: 10.3390/ijms22041895

Figure 8.

Figure 8

Melanogenesis-related molecules are mediated by YAP. (A,B) B16F10 cells were transfected with a plasmid overexpressing either GFP (a control) or YAP, and incubated for a further 24 h. (A) pYAP, YAP, MITF, TYR, and TYRP-1, 2 levels were evaluated by Western blotting. β-actin was used as a loading control. (B) YAP, MITF, TYR and TYRP-1, 2 expression levels were determined by qRT-PCR. (C,D) B16F10 cells were transfected with either control siRNA or YAP siRNA, and incubated for 24 h. (C) pYAP, YAP, MITF, TYR, TYRP-1, 2 and β-actin were determined by Western blotting, and (D) YAP, MITF, TYR and TYRP-1, 2 expression levels were analyzed by qRT-PCR. Data represent three independent experiments (means ± SD). * p < 0.05, ** p < 0.01, *** p < 0.001. Numerical values on the blots represent DU. The control was normalized to 1 DU.