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. 2021 Feb 14;10(2):392. doi: 10.3390/cells10020392

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Accumulation of WBNV in ligaments connecting PSG to anterior midgut during P2 stage of Thrips palmi. (A). Alexa Fluor 594 phalloidin-conjugated F-actin probe red fluorescence (B). FITC-conjugated goat anti-rabbit secondary antibody green fluorescence indicating WBNV-N protein (C). Merged image of red and green fluorescence signals. MG1—midgut 1; Lg—ligament. Ligaments connecting midgut to PSG were intact during pupal stages. WBNV infection was detected in ligaments connecting MG1 to PSG.