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. 2021 Feb 14;10(2):392. doi: 10.3390/cells10020392

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Accumulation of WBNV in anterior midgut of Thrips palmi adult. (A). Alexa Fluor 594 phalloidin-conjugated F-actin probe red fluorescence (B). FITC-conjugated goat anti-rabbit secondary antibody green fluorescence indicating WBNV-N protein (C). Merged image of red and green fluorescence signals. FG—foregut; MG1—midgut 1. Level of WBNV infection was highest in anterior midgut (top panel). Green fluorescence indicating WBNV N protein increased in midgut 12 days post acquisition. Infection of longitudinal and circular muscle cells of the midgut was evident by presence of green fluorescence (middle panel). No FITC green fluorescence was observed in the midgut of aviruliferous T. palmi (bottom panel).