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. 2021 Feb 14;9(2):384. doi: 10.3390/microorganisms9020384

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of each group of amino acids on the regrowth of X. citri persister cells (A) amino acids group #1 (Ala, Arg, Cys, Phe, Ser); group #2 (Gly, His, Thr, Val, Tyr); group #3 (Asn, Ile, Lys, Pro, Trp) and group #4 (Asp, Glu, Gln, Leu, Met). (B) Same experiment as shown in A, but with M9 minimal medium containing individual amino acids of the fast-growing group #3 and group #4. Photographs show colonies observed with proline (group #3), glutamine (group #4) and aspartic acid (group #4). (C) To observe the inhibitory effect of isoleucine on X. citri persisters, a suspension of X. citri persister cells (same experiment as in A) were plated on M9-agar plates supplemented with only isoleucine; isoleucine + proline or only proline.

Effect of each group of amino acids on the regrowth of X. citri persister cells (A) amino acids group #1 (Ala, Arg, Cys, Phe, Ser); group #2 (Gly, His, Thr, Val, Tyr); group #3 (Asn, Ile, Lys, Pro, Trp) and group #4 (Asp, Glu, Gln, Leu, Met). (B) Same experiment as shown in A, but with M9 minimal medium containing individual amino acids of the fast-growing group #3 and group #4. Photographs show colonies observed with proline (group #3), glutamine (group #4) and aspartic acid (group #4). (C) To observe the inhibitory effect of isoleucine on X. citri persisters, a suspension of X. citri persister cells (same experiment as in A) were plated on M9-agar plates supplemented with only isoleucine; isoleucine + proline or only proline.