Table 1.
1. Platelet-Rich Plasma Preparation | |
Initial blood volume | 10 mL |
Anticoagulant | Sodium citrate 3.8% (wt/V) |
System | Close |
Centrifugation | Yes |
number | 1 |
speed | 580× g—8 min |
Final PRP volume | 4 mL per subject |
2. Platelet-Rich Plasma Characteristics | |
PRP Type | 11-00-11 |
Mean Platelet Volume (MPV) | 9.6 ± 0.6 fL |
Red Blood Cells | <0.01 × 106/µL |
White Blood Cells | <0.05 × 106/µL |
Neutrophils | --- |
Lymphocytes | --- |
Monocytes | --- |
Eosinophils | --- |
Basophils | --- |
Activation | CaCl2 (10% wt/vol) |
3. Application Characteristics | |
Dose | 2% |
Direct/Indirect | Direct |
Cell line | Lung fibroblast |
4. Other Remarkable Platelet-Rich Plasma and Study Features | |
The product added to the cell cultures was the platelet lysate obtained after activation of PRP with calcium chloride (10%) |