Figure S1.

Approach and validation of matrix stiffness in regulating chromatin accessibility. (a) Schematic displaying approach to isolate mouse lung GFP⁺ fibroblasts. Single-cell suspensions were first depleted of dead cells, followed by CD45, CD326, and CD31 depletion, and then followed by a GFP⁺ selection. Cells were then plated on collagen I–coated tissue-culture plastic and 0.2-kPa PDMS for 8 d. Cells were then submitted for ATAC-seq. (b) Volcano plot of DASs of fibroblasts on 0.2-kPa versus 32-kPa PDMS matrices. Each dot represents a single accessibility site. Significantly different accessibility sites colored in blue. (c) De novo DNA motif enrichment analysis of differential chromatin accessibility loci of fibroblasts on 0.2-kPa versus 32-kPa PDMS matrices ranked by P value. “% of targets” indicates the percentage of total input sequences that contain that respective motif.