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. 2021 Feb 12;22(4):1825. doi: 10.3390/ijms22041825

Figure 6.

Figure 6

Effect of Bam32 deficiency on CXCL2-induced phosphorylation of Akt in neutrophils. (A) The levels of phospho-Akt1 (p-Akt1) at Ser473 and total Akt1 (t-Akt1), as well as the relative expression determined in bone marrow-derived WT and Bam32−/− neutrophils treated for 5 min with saline or CXCL2 (0.1 µM). (B) Representative original Western blots and the levels of phospho-Akt2 (p-Akt2) at S474, phospho-Akt1/2 (p-Akt1/2) at Thr308/309, total Akt2 (t-Akt2), and the relative expression of phospho-Akt2 (p-Akt2) at S474 over total Akt2 (t-Akt2) determined in bone marrow-derived WT and Bam32−/− neutrophils stimulated for 5 min with CXCL2 (0.1 µM) and with the treatment of DMSO (vehicle) or GGTI298 (10 µM) for 30 min prior to and throughout the 5-min CXCL2 stimulation. (A,B): mean ± SEM of 3 samples per group. Each sample was individually collected and pooled from two mice of the same strain. * indicates significant difference between the two groups (p < 0.05).