Skip to main content
. 2021 Feb 19;4(5):e202000974. doi: 10.26508/lsa.202000974

Figure S2. Transcriptome analysis of the effects of the transcription factor expression.

Figure S2.

(A) (left) Principal component analysis of the expression of the 481 genes characterizing human primordial germ-cell–like cell specification shown in reference 13. Red (first quadrant): genes for PGCLC specification; cyan (fourth quadrant): genes for endoderm/mesoderm specification; yellow (second and third quadrants): genes for pluripotency (right). Heat map representation of the expression of the 481 genes in the parental hiPSCs, iMeLCs, bone morphogenetic protein (BMP)4-induced d1 whole aggregates, and d2/d4 BT+AG+ cells, and in TFAP2C/BLIMP1, SOX17/TFAP2C, SOX17/BLIMP1, SOX17/TFAP2C/BLIMP1 clone-derived, Dox-induced d2 BT+ cells. Representative genes are annotated, and the correlation coefficient values of each induction with the d4 BT+AG+ cells are shown. The color coding is as indicated. (B, C) Expression dynamics of the genes up-regulated in BMP-stimulated (black, BT+AG+) compared with Dox-stimulated (red, BT+) d2 cells (B) (Note that TFAP2A, HAND1, CDX2, HAPLN1, and MSX2 were highly up-regulated also at d1 [Fig 2H]) or those up-regulated in Dox-stimulated (red) compared with BMP-stimulated (black) SOX17/TFAP2C/BLIMP1 clone-derived d1 iMeLC aggregates (C). The bars indicate the mean value of each time point, and the ranks of the genes ordered by the fold changes between BMP- and Dox-stimulation are shown. See Table S1 for the samples analyzed. (D, E) Gene ontology terms (blue) and KEGG pathways (pink) enriched in differentially expressed genes between BMP and Dox- and Dox-stimulated SOX17/TFAP2C clone-derived cells at d2 (BMP and Dox: BT+AG+; Dox: BT+). (D, E) Representative genes up-regulated in BMP and Dox- (D) or Dox- (E) stimulations and P-values are shown.