Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| BTAG (BLIMP1-tdTomato and TFAP2C-eGFP knockin reporters in the 585B1 hiPSCs) | Sasaki et al (2015). | N/A |
| BTAG; SOX17 OE#1 | This study | N/A |
| BTAG; SOX17 OE#2 | This study | N/A |
| BTAG; TFAP2C OE#1 | This study | N/A |
| BTAG; TFAP2C OE#3 | This study | N/A |
| BTAG; SOX17+BLIMP1 OE#9 | This study | N/A |
| BTAG; SOX17+BLIMP1 OE#15 | This study | N/A |
| BTAG; TFAP2C+BLIMP1 OE#2 | This study | N/A |
| BTAG; TFAP2C+BLIMP1 OE#15 | This study | N/A |
| BTAG; SOX17+TFAP2C OE#2 | This study | N/A |
| BTAG; SOX17+TFAP2C OE#11 | This study | N/A |
| BTAG; SOX17+TFAP2C OE#28 | This study | N/A |
| BTAG; SOX17+TFAP2C+BLIMP1 OE#4 | This study | N/A |
| BTAG; SOX17+TFAP2C+BLIMP1 OE#9 | This study | N/A |
| BTAG; SOX17+TFAP2C+BLIMP1 OE#22 | This study | N/A |
| BTAG; SOX17+TFAP2C+BLIMP1 OE#28 | This study | N/A |
| BTAG; SOX17+TFAP2C+BLIMP1 OE#31 | This study | N/A |
| BTAG; SOX17+TFAP2C+MSX2 OE#7 | This study | N/A |
| BTAG; SOX17+TFAP2C+MSX2 OE#9 | This study | N/A |
| BTAG; GATA3+SOX17+TFAP2C OE#1 | This study | N/A |
| BTAG; GATA3+SOX17+TFAP2C OE#5 | This study | N/A |
| BTAG; GATA2+SOX17+TFAP2C OE#29 | This study | N/A |
| BTAG; GATA2−/− #1 | This study | N/A |
| BTAG; GATA2−/− #6 | This study | N/A |
| BTAG; GATA2−/− #12 | This study | N/A |
| BTAG; GATA3−/− #17 | This study | N/A |
| BTAG; GATA3−/− #18 | This study | N/A |
| BTAG; GATA3−/− #30 | This study | N/A |
| BTAG; GATA3−/− #40 | This study | N/A |
| BTAG; HAND1−/− #6 | This study | N/A |
| BTAG; GATA3−/−; GATA2+/− #5-1 | This study | N/A |
| BTAG; GATA3−/−; GATA2−/− #5-10 | This study | N/A |
| BTAG; GATA3−/−; GATA2−/−; GATA3 OE #19 | This study | N/A |
| Recombinant DNA | ||
| pX335-U6-Chimeric BB-CBh-hSpCas9n (D10A) | Addgene | Cat. no. 42335 |
| Antibodies | ||
| Goat anti-SOX17 | R&D Systems | AF1924; RRID: AB_355060 |
| Mouse anti-TFAP2C | Santa Cruz | sc-12762; RRID: AB_667770 |
| Mouse anti-BLIMP1 | R&D Systems | MAB36081; RRID: AB_10718104 |
| Mouse anti-GATA3 | BIOCARE | ACR405A; RRID: AB_10895444 |
| Rabbit anti-GATA3 | Cell Signaling | CST5852S; RRID:AB_10835690 |
| Rabbit anti-GATA2 | Novus | NBP82581; RRID:AB_11026191 |
| Rabbit anti-GATA2 | Santa Cruz | sc9008; RRID:AB_2294456 |
| Mouse anti-human mitochondria | Merck Millipore | MAB1273; RRID:AB_94052 |
| Goat anti-DDX4 | R&D Systems | AF2030; RRID:AB_2277369 |
| Mouse IgG – HRP conjugated | Sigma-Aldrich | A5906; RRID: AB_258264 |
| Mouse anti-α Tubulin | Sigma-Aldrich | T9026; RRID: AB_477593 |
| Mouse IgG – HRP conjugated | Sigma-Aldrich | A5906; RRID: AB_258264 |
| Rabbit IgG – HRP conjugated | Sigma-Aldrich | A6154; RRID: AB_258284 |
| Goat IgG – HRP conjugated | Sigma-Aldrich | A5420; RRID: AB_258242 |
| SSEA1 (CD15) microbeads for human and mouse | Miltenyi Biotec | 130-094+530 |
| CD31 microbeads for mouse | Miltenyi Biotec | 130-097-418 |
| Oligonucleotides and sequence-based reagents | ||
| qRT-PCR primers | This study | Table S3 |
| Chemicals, enzymes and other reagents | ||
| StemFit AK03N | Ajinomoto | N/A |
| iMatrix-511 | Nippi | |
| Puromycin | Thermo Fisher Scientific | A1113803 |
| G418, Geneticin | Thermo Fisher Scientific | #10131035 |
| Hygromycin B | Thermo Fisher Scientific | #10131035 |
| Doxycycline | Takara-Clontech | Z1311N |
| Fibronectin | Millipore | FC010 |
| GMEM | Thermo Fisher Scientific | #11710035 |
| Knockout serum replacement | Thermo Fisher Scientific | A3181502 |
| Activin A | Peprotech | 120-14E |
| CHIR99021 | TOCRIS | #4423 |
| Y27632 | FujiFilm | 030-24021 |
| BMP4 | R&D Systems | 314-BP |
| SCF | R&D Systems | 255-SC |
| EGF | R&D Systems | 236-EG |
| LIF | Millipore | LIF1010 |
| LDN193189 | StemGent | 04-0074 |
| Glutamax | Thermo Fisher Scientific | 35050-061 |
| HEPES | Thermo Fisher Scientific | 15630-106 |
| α-Minimum Essential Medium | Thermo Fisher Scientific | 32571-036 |
| L-ascorbic acid | Sigma-Aldrich | A4403 |
| Software | ||
| FACSDiva Software | BD Biosciences | N/A |
| DAVID (v6.8; GO analysis) | https://david.ncifcrf.gov/ | N/A |
| FV10-ASW | Olympus | N/A |
| R (v3.6.0; PCA, DEG, and graphs) | https://www.R-project.org | N/A |
| Bowtie2 v2.2.7 | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml | N/A |
| TopHat v2.1.0 | https://ccb.jhu.edu/software/tophat/index.shtml | N/A |
| HTSeq v0.9.1 | https://htseq.readthedocs.io/en/master/overview.html | N/A |
| ImageJ/Fiji | Fiji.sc | N/A |
| Trim_galore v0.6.3 | https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ | N/A |
| cutadapt v118 | http://cutadapt.readthedocs.io/en/stable/guide.html | N/A |
| Bismark v0.22.1 | https://www.bioinformatics.babraham.ac.uk/projects/bismark/ | N/A |
| SAMtools v1.9 | http://samtools.source-forge.net | N/A |
| Other | ||
| pGEM-T Easy Kit | Promega | A3600 |
| Gateway LR Clonase Enzyme Mix | Thermo Fisher Scientific | #11791043 |
| v-bottom 96-well plate | Greiner | #651970 |
| RNeasy Micro Kit | QIAGEN | #74004 |
| NucleoSpin RNA XS | Macherey-Nagel | #740902 |
| Qubit RNA HS assay kit | Thermo Fisher Scientific | Q32855 |
| PowerSYBR Green PCR Master Mix | Thermo Fisher Scientific | #4367659 |
| Qubit dsDNA HS assay kit | Thermo Fisher Scientific | Q32851 |
| Protein Quantification Assay | Macherey-Nagel | #740967 |
| ECL Western Blotting Detection Reagent | GE Healthcare Life Sciences | RPN2106 |
| EZ DNA Methylation-Gold Kit | Zymogen | D5005 |
| DP72 | Olympus | N/A |
| FV1000-IX81 confocal microscope system | Olympus | N/A |
| CFX384 Touch Real-Time PCR detection system | Bio-Rad Laboratories | N/A |
| NextSeq500/550 | Illumina | N/A |
| Hiseq2500 | Illumina | N/A |