Figure 3.

The synonymous BAP1 mutation leads to exon skipping

(A) Diagram of the bichromatic alternative splicing reporter system to assess exon inclusion or skipping.

(B–D), BAP1 exon 11 is skipped in HeLa cells transiently transfected with a minigene reporter containing the c.936T>G, p.G312G synonymous mutation whereas it is more inclusive with the wild-type exon 11, as indicated by western blotting of Flag-tagged proteins (B), RT-PCR (C) and fluorescence microscopy (D). Quantifications are the average ± SD of three independent experiments. ∗, p<0.05; ∗∗, p<0.01; ∗∗∗, p<0.001. Scale bar represents 200 µm.