Figure 2.

Flowchart of the search for species-specific sequences. The workflow includes three stages. Stage I is the preparation stage and consists of collecting the genomes, creating BLAST databases and splitting the target genome into sequences of 100 bases in length, overlapping by 90 bases, for performing BLAST searches in subsequent stages. Stage II is the search stage and consists of BLAST searches using negative and positive group databases to reveal possible species-specific regions. Stage III is the analysis stage and can include the analysis of the genome map, a BLAST search using other databases and choosing the most appropriate sequences for PCR purposes, and so on.