Table 2.
Mean Cq values for qPCR carried out on serial dilutions of genomic DNA of the P. atrosepticum SCRI1043 and corresponding plasmid.
| Plasmid DNA per Reaction | Genomic DNA per Reaction | |||||
|---|---|---|---|---|---|---|
| № | Plasmid Copies |
Mean Cq |
Standard Deviation | Genome Copies |
Mean Cq | Standard Deviation |
| 1 | 3.7 × 109 | 7.03 | 0.96 | 8.7 × 105 | 19.35 | 0.83 |
| 2 | 3.7 × 108 | 9.98 | 0.54 | 8.7 × 104 | 21.82 | 0.77 |
| 3 | 3.7 × 107 | 12.02 | 1.28 | 8.7 × 103 | 24.26 | 0.40 |
| 4 | 3.7 × 106 | 16.58 | 0.35 | 8.7 × 102 | 29.67 | 1.40 |
| 5 | 3.7 × 105 | 20.01 | 0.51 | 87 | 33.3 | 2.40 |
| 6 | 3.7 × 104 | 22.95 | 1.02 | 8.7 | - | - |
| 7 | 3.7 × 103 | 26.75 | 0.95 | 0.87 | - | - |
| 8 | 3.7 × 102 | 30.05 | 0.33 | 0.087 | - | - |