Reagent	Stock concentration	Work concentration	Volume per 10 ml
Dextran sulphate	50%	10%	2 ml
Ribonucleoside–vanadyl complex	200 mM	10 mM	0.5 ml
SSC buffer	20 fold	2 fold	1 ml
Sodium phosphate buffer pH 7	1 M	50 mM	0.5 ml
Formamide	100%	50%	5 ml
DEPC-treated water	n/a	n/a	1 ml
		Total = 10 ml