Figure 7. Candida-induced renal IFNγ is not produced by classical IFNγ-producing cells.

IFNγ-producing cells were analyzed in infected WT and CD169-DTR kidneys at day 6 p.i. (A) Contour plot of kidney cells stained for IFN-γ and CD45 (left). Expression profile of a panel of surface markers by IFNγ⁺ cells (red dots) and IFNγ⁻ cells (blue dots). IFNγ⁺ cells can be identified as Ly6C^intLyG^lo, F4/80^loCD11b^lo, MHCII⁺CD11c^lo, CD49b^negCD3^neg, kappa-light chain⁺CD19^int, and CD8^negCD4^neg. (B) Absolute number of IFNγ⁺ kappa-light chain⁺CD19^int cells in kidneys isolated from uninfected, infected, control and CD169-DTR mice (day 6 p.i.). t test (two-tailed). *P < 0.05.