Table 1.
Major GSLs and SM of pHRCEpiCs determined by mass spectrometry combined with TLC immunodetection a.
| Compound b | Ceramide | Formula | m/zexpc | m/zcalcc |
|---|---|---|---|---|
| SM | d18:1, C16:0 | C39H79N2O6PNa | 725.56 | 725.5573 |
| MHC | d18:1, C16:0 | C40H77NO8Na | 722.55 | 722.5547 |
| MHC | d18:1, C20:0 | C44H85NO8Na | 778.61 | 778.6173 |
| MHC | d18:1, C22:0 | C46H89NO8Na | 806.65 | 806.6486 |
| MHC | d18:1, C24:1 | C48H91NO8Na | 832.67 | 832.6642 |
| MHC | d18:1, C24:0 | C48H93NO8Na | 834.67 | 834.6799 |
| Lc2Cer | d18:1, C16:0 | C46H87NO13Na | 884.60 | 884.6075 |
| Lc2Cer | d18:1, C22:0 | C52H99NO13Na | 968.70 | 968.7014 |
| Lc2Cer | d18:0, C24:1 | C54H101NO13Na | 994.72 | 994.7171 |
| Lc2Cer | d18:0, C24:0 | C54H103NO13Na | 996.73 | 996.7327 |
| Gb3Cer | d18:1, C16:0 | C52H97NO18Na | 1046.67 | 1046.6603 |
| Gb3Cer | d18:1, C22:0 | C58H109NO18Na | 1130.76 | 1130.7542 |
| Gb3Cer | d18:1, C24:1 | C60H111NO18Na | 1156.78 | 1156.7699 |
| Gb3Cer | d18:1, C24:0 | C60H113NO18Na | 1158.78 | 1158.7855 |
| Gb4Cer | d18:1, C16:0 | C60H110N2O23Na | 1249.74 | 1249.7397 |
| Gb4Cer | d18:1, C22:0 | C66H122N2O23Na | 1333.83 | 1333.8336 |
| Gb4Cer | d18:1, C24:1 | C68H124N2O23Na | 1359.85 | 1359.8493 |
| Gb4Cer | d18:1, C24:0 | C68H126N2O23Na | 1361.87 | 1361.8649 |
| Gb5Cer | d18:1, C16:0 | C66H120N2O28Na | 1411.79 | 1411.7925 |
| Gb5Cer | d18:1, C22:0 | C72H132N2O28Na | 1495.89 | 1495.8864 |
| Gb5Cer | d18:1, C24:1 | C74H134N2O28Na | 1521.91 | 1521.9021 |
| Gb5Cer | d18:1, C24:0 | C74H136N2O28Na | 1523.92 | 1523.9177 |
a The neutral GSL preparation was obtained from replicate 1 of pHRCEpiCs; TLC overlay detection of Gb3Cer and Gb4Cer was performed with Stx1a and Stx2a as well as anti-Gb3Cer and anit-Gb4Cer antibodies (see Figure 1); b all sphingolipids were detected in the positive ion mode as monosodiated [M+Na]+ species; exemplary MS2 spectra of Gb3Cer (d18:1, C16:0), Gb4Cer (d18:1, C22:0), and proposed Gb5Cer (d18:1, C16:0) are provided in Figure S2, Figure S3, and Figure S4, respectively, of the Supplementary Materials; c exp, experimental; calc, calculated.