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. 2021 Jan 28;10(2):252. doi: 10.3390/cells10020252

Figure 2.

Figure 2

Decreased liver fibrosis progression in Mif-deficient mice in an experimental NASH model. (A) Quantification and (B) representative images of sirius red staining of WT and Mif−/− mice after 8 weeks of MCD feeding. (C) Decreased fibrosis in Mif−/− mice compared to WT (n = 10 per group) was confirmed by significantly decreased concentrations of hydroxyproline within the liver. (D) Immunhistochemical stainings for α-SMA in liver samples of WT and Mif−/− mice after 8 weeks of MCD feeding. (E) Immunoblot analysis on whole liver tissue of WT and Mif−/− mice after 8 weeks of MCD feeding using antibodies against α-SMA, tubulin served as loading control. (F) Relative α-SMA protein level. (G) Relative Acta2 mRNA expression in total liver tissue of WT and Mif−/− mice after 8 weeks of MCD feeding (n = 10 per group) quantitated using qRT-PCR (fold induction normalized to control animals). (H) Expression patterns of fibrosis-related genes, e.g., Col1a1, Timp1, Mmp2, Mmp9, and Tgf-β1, were measured by qRT-PCR between Mif+/+ mice and Mif−/− mice (n = 10 per group). Asterisks indicate statistical significance: * p < 0.05; ** p < 0.01; *** p < 0.001.