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SEC-FPLC purification of the A3BCTD* multimers. (A) SEC-FPLC of A3BCTD* run through a SEC column at a flow rate of 0.5 mL/min at 25 °C, displaying the elution of two forms of A3BCTD*, peak 1 and peak 2. (B) 16% Tricine SDS-PAGE gel (reducing conditions) of the purified A3BCTD* fractions after SEC purification.
