Table 2.
SAXS fitting and modelling parameters of the A3BCTD* and A3BCTD*/dZ-oligo complex.
| Modelling Parameters | A3BCTD* | A3BCTD*/dZ-Oligo | A3BCTD* Dimer |
|---|---|---|---|
| Ab initio restoration | |||
| DAMMIF a | |||
| q range (Å−1) for fitting | 0.011–0.354 | 0.011–0.354 | 0.0016–0.345 |
| Symmetry | 1 (none) | 1 (none) | P2 |
| NSD (standard deviation) a | 0.559 (0.019) | 0.862 (0.037) | 0.816 (0.110) |
| Resoln (from SASRES) (Å) | 28 (2) | 29 (2) | 41 ± 3 |
| Structure modelling | |||
| q range for modelling (Å−1) | 0.011–0.302 | 0.011–0.302 | |
| PDB structure | 5CQI | 5TD5* b | Interface model 1 |
| FoXS, CRYSOL (Chi2) | 1.16, 1.99 | 1.51, 2.08 | 0.93, 1.49 |
| Interface model 2 | |||
| FoXS, CRYSOL (Chi2) | -- | -- | 0.80, 1.53 |
a Default parameters, 10 calculations; NSD explained in main text. b Structure 5TD5* is the 5TD5 structure in which the length of ssDNA was increased from a 5- to a 9-mer.