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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Epilepsia. 2020 Sep 17;61(10):2301–2312. doi: 10.1111/epi.16670

Figure 6. ER stress increased TNF in cultured cells but the cells expressing the mutant γ2(Q390X) had dampened response.

Figure 6.

A. The forebrain cortex of postnatal day 0 old pups of Gabrg2+/Q390X and Gabrg2+/− mouse lines were dissected, disassociated and the neurons were cultured for 15 days in dish before harvest. Equal amounts of cell protein lysates (30μg) from the wildtype or the heterozygous mice were determined for TNF with enzyme-linked immunosorbent assay (ELISA). The measurements in the het were normalized to the wildtype sister cultures. B. Mouse L929 cells were transfected with the wildtype or the mutant γ2(Q390X) subunits in combination with the wildtype α1 and β2 subunits for 48 hrs. Sister cultures were treated with or without tunicamycin (10μg/ml) for 16 hrs before harvest. Equal amounts of cell protein lysates (30μg) from each group were determined for TNF with ELISA. (**p < 0.01 vs wt, ***p < 0.001 vs wt, § p<0.05 vs Wt+Tuni. n = 4–5 batches of cultures. Data were presented as mean ± S.E.M).