Figure 2.

Functionality of OVA-specific T cells as determined by in vivo cytolytic assay in immunized mice. C57BL/6 mice (n = 5/group) were immunized i.m. with OVA SLP (30 µg) with or without adjuvant on days 0, 7, and 14. On day 19, mice were administered CFSE-labeled cells that had been pulsed with the OVA CD8⁺ T cell epitope, SIINFEKL. Splenocytes were harvested on day 20 and analyzed for in vivo cytolytic activity (A) or by IFN-γ ELISpot when stimulated by OVA CD8⁺ T cell peptide epitope SIINFEKL (CFSE: Carboxyfluorescein succinimidyl ester) (B) or OVA CD4⁺ T cell peptide epitope TEWTSSNVMEER (C). For ELISpot, values obtained with media alone were subtracted from those measured in the presence of the peptides. Grouped data are presented as mean + standard error of mean (SEM). Statistical significance of differences for OVA SLP + SLA + Poly(I:C) vs. other groups is shown: *** p < 0.001 and **** p < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparisons test.