Effect of BZ and CFZ on cell viability of various melanoma cells. (A,B) B16-F1, B16-BL6, K-1735, DX3, A375P, and A375SM were treated with a vehicle or various concentrations of BZ (A) or CFZ (B) for 2 days in the culture medium. Cell viability was assessed by MTT assay and the percent viabilities were calculated (n = 12 for total replicates). The regression fit curves and dots corresponding to the drug concentration as average ± standard deviation are plotted using Sigma Plot. (C) The morphological changes were observed by an inverted microscope after treatment with 10 and 50 nM BZ or CFZ for 2 days, and the 100× magnified photos with 100 µm scale bar are shown.