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. 2021 Feb 15;10(2):187. doi: 10.3390/antibiotics10020187

Figure 1.

Figure 1

Effect of Bacteroides species on Clostridium difficile (CD) toxin production. Cytopathic effect by CD toxins A and B in the culture supernatants were evaluated by a cytotoxicity assay using (a) human colon cancer cell line HT-29 cells and (b) Vero cells. CD, CD ATCC9689; CD/BF, co-culture of CD ATCC9689 and B. fragilis YCH46; CD/BT, co-culture of CD ATCC9689 and B. thetaiotaomicron VPI-5482; and N, GAM broth only. Western blotting of the cell-free CD supernatants in the conditioned media with either (c) anti-Toxin A or (d) anti-Toxin B antibody. CD, CD ATCC9689 culture supernatant in GAM broth; conditioned medium containing 50% (v/v) culture supernatant of BT, B. thetaiotaomicron VPI-5482; BF, B. fragilis YCH46; BO, B. ovatus ATCC8483; BU, B. uniformis ATCC8492; BV, B. vulgatus ATCC8503; BL, Bifodobacterium longum JCM1217; and CR, Clostridium ramosum JCM1298. M, HPR-labelled protein size marker.