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Characterization of the ΔgcpE mutant strain. (a) The Tn4351 insertion site in the ΔgcpE mutant. Tn4351 was inserted into the 5′ end of gcpE. * Stop codon of the upstream gene (BT2518). (b) Gram stain of wild type BT and ΔgcpE strains. (c) Mucoid layer in BT culture following centrifugation of either BT WT or ΔgcpE. (d) Toxin A levels in CD culture alone, exposed to the supernatant of wildtype BT or ΔgcpE.
