Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 15;10(2):187. doi: 10.3390/antibiotics10020187

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effect of BT-derived PFs on PaLoc gene expression. After CD was cultured to late-logarithmic phase in the conditioned medium containing 2.0 mg/mL PFs from the indicated strains, the RNA was extracted from each sample. RT-qPCR was performed to examine the change in gene expression for toxins A and B (tcdA and tcdB), the positive- and negative-regulators (tcdR and tcdC), and the holin-like protein (tcdE). The relative expression of each gene to the control (expression in the conditioned medium prepared with PF from GAM broth) is shown. Significant difference compared with GAM is indicated by * (p < 0.01).