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Localization of Toxin A in CD culture. After CD was cultured in the conditioned medium containing PF (2.0 mg/mL) derived from GAM broth or BT, the culture was separated into the supernatant and cell pellet by centrifugation. Toxin A levels in the supernatant (S) and cell pellet (P) were evaluated by Western blotting with anti-Toxin A antibody. M, HRP-labeled protein size marker.
