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. 2021 Feb 15;10(2):187. doi: 10.3390/antibiotics10020187

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lysozyme treatment reduced the suppressive effect of BT-derived PF on CD toxin production. PFs from either GAM broth or the culture supernatants of B. thetaiotaomicron VPI-5482 (BT), Tn4351-inserted gcpE mutant of BT (ΔgcpE), or B. uniformis ATCC8492 (BU) were treated with lysozyme. CD Toxin A production in the conditioned media, which contained PFs with or without lysozyme treatment (indicated by + and -, respectively), was compared. CD, CD culture supernatant in GAM broth; M, HRP-labeled protein size marker.