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. 2021 Feb 15;13(4):807. doi: 10.3390/cancers13040807

Figure 1.

Figure 1

The proliferation phenotypes of various human mammary epithelial cells in adherent culture or in anchorage-independent soft-agar growth condition. (A) Proliferation of human mammary epithelial (HME) cells at different stages of transformation cultured under adherent condition. HME-hTERT: immortalized human mammary epithelial cells; HME-ST: HME-hTERT also expressing SV40 small T antigen. Each isoform of constitutively active RAS was introduced into the two cell lines; grey lines and black lines represent the cells with or without the expression of the indicated isoforms of constitutively active RAS: CA-NRAS, CA-HRAS and CA-KRAS. (B) Representative microscopic images documenting colony formation of the indicated HME cells, as in panel A, cultured in soft agar for 11 days. (C) Quantitation of the data from (B), summed from three representative microscopic fields for each condition. Data are presented as mean ± SD; *** p ≤ 0.001 (Student’s t-test). All studies presented above have been repeated twice with similar results.