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. 2021 Mar 1;23:7. doi: 10.1186/s12575-021-00143-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Fluorescence imaging with Fura-2 and CTR. Fura-2 is diluted in one well (a), then a 1 ml aliquot is moved to an adjacent well and CTR is added (b). Islets are added to each well and incubated for 30 min (c) before being combined in the Fura-2 only well and transferred to the recording chamber (d). Islets are imaged using three different wavelengths: bright field (e), CTR only (f), and Fura-2 (g). Once the calcium imaging experiment is complete, the islets from the second well can be identified as the red islets in the CTR image