Fig. 3.

CircWAC decrease chemosensitivity to PTX in TNBC. a, b The efficiency of circWAC overexpression and silencing in MDA-MB-231 and MDA-MB-468 cells was verified by qRT-PCR. c-f Cells were treated with PTX (concentrations of 0, 5, 10, 15, 20, 25 and 30 μmol/L) for 24 h, and cell viability was evaluated by a CCK-8 assay. g-j Cells were treated with paclitaxel (0 and 20 μM) for 24 h, and cell viability was evaluated by a colony formation assay. k, l Cells were treated with paclitaxel (0 and 20 μM) for 24 h, and the apoptosis rate was analyzed via flow cytometry. The data are presented as the means±S.D. of at least three independent experiments. m The expression of circWAC was significantly increased in the chemotherapy resistance patients. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001