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. 2021 Mar 1;20:43. doi: 10.1186/s12943-021-01332-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — CircWAC acts as a sponge of miR-142 in TNBC. a The expression level of miR-142 in MDA-MB-231 and MDA-MB-468 cells transfected with circWAC or control. b The expression level of miR-142 in MDA-MB-231 and MDA-MB-468 cells transfected with si-circWAC or si-NC. c FISH showed that circWAC and miR-142 were colocalized in the cytoplasm of MDA-MB-231 cells. d The coprecipitated miR-142 was subjected to qRT-PCR to assess circWAC binding by RIP experiments in MDA-MB-231 cells. e MiR-142 and wild-type/mutant circWAC binding site sequences. f Luciferase activity in MDA-MB-231 cells cotransfected with a luciferase reporter vector containing the wild-type or mutant circWAC sequence and the miR-142 mimic or control. g The expression of miR-142 was negatively correlated with circWAC in 90 TNBC patients. h The expression level of miR-142 in TNBC tissues and adjacent nontumor tissues was measured by qRT-PCR. Kaplan-Meier survival analysis of patients with TNBC stratified by the level of miR-142 were used to evaluate OS based on our dataset (N = 90) (I), a METABRIC dataset (N = 203) (J) and a TCGA dataset (N = 97) (K)