Fig. 7.

MiR-142 increases PTX sensitivity and inhibits the PI3K/AKT signaling pathway by targeting WWP1. a The protein levels of WWP1, PTEN, AKT and pS473-AKT in MDA-MB-231 and MDA-MB-468 cells transfected with control + miR-NC, control + miR-142 mimic, WWP1 + miR-NC, or WWP1 + miR-142 mimic were measured by Western blotting. b, c MDA-MB-231 and MDA-MB-468 cells transfected with control + miR-NC, control + miR-142 mimic, WWP1 + miR-NC, or WWP1 + miR-142 mimic were treated with PTX (concentrations of 0, 5, 10, 15, 20, 25 and 30 μmol/L) for 24 h, and cell viability was evaluated by a CCK-8 assay. d MDA-MB-231 and MDA-MB-468 cells transfected with control + miR-NC, control + miR-142 mimic, WWP1 + miR-NC, or WWP1 + miR-142 mimic were treated with 20 μM paclitaxel for 24 h, and cell viability was evaluated by a colony formation assay. e MDA-MB-231 and MDA-MB-468 cells transfected with control + miR-NC, control + miR-142 mimic, WWP1 + miR-NC, or WWP1 + miR-142 mimic were treated with 20 μM paclitaxel for 24 h, and the apoptosis rates were analyzed via flow cytometry. The data are presented as the means±S.D. of at least three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001