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hsa-miR-9-5p down regulates HK2 expression. (A) The results of the dual luciferase reporter gene system showed that hsa-miR-9-5p directly targeted to HK2. (B) The effects of hsa-miR-9-5p on HK2 mRNA was determined using qRT-PCR. HK2 mRNA was significantly reduced by treatment with miR-9-5p agomir. (C) The effects of hsa-miR-9-5p on HK2 protein expression was determined using Western blot. HK2 protein was significantly inhibited by miR-9-5p agomir (** p < 0.01 vs. miR-NC).
