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. 2021 Mar 1;7:43. doi: 10.1038/s41420-021-00428-w

Fig. 4. Atazanavir prevented capase-1-dependent lytic monocyte death.

Fig. 4

Human monocytes were infected with SARS-CoV-2 and treated with atazanavir (ATV; 10 µM), ribavirina (10 µM), or Lopinavir (10 µM) for 24 h. A, B Monocytes were stained with FAM-YVAD-FLICA to determine caspase-1 activity. C Monocytes were stained with FAM-FLICA to determine caspase-3/7 activity. D–G Culture supernatants were collected and the levels of IL-1β, IL-6, TNF-α, and IL-8 were determined by ELISA. H Assessment of cell viability through the measurement of LDH levels in the supernatant of monocytes. Histogram and graphs data are representative of six independent experiments. Data are presented as the mean ± SEM * P < 0.05 versus control group (MOCK-infected); #P < 0.05 versus only infected group.